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超高频超声成像分析黑色素瘤Braf/Pten基因工程小鼠模型中的淋巴结体积

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JoVE Journal Cancer Research

Analysis of Lymph Node Volume by Ultra-High-Frequency Ultrasound Imaging in the Braf/Pten Genetically Engineered Mouse Model of Melanoma

Please note that all translations are automatically generated. Click here for the English version.

超高频超声成像分析黑色素瘤Braf/Pten基因工程小鼠模型中的淋巴结体积

DOI:

10.3791/62527-v

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08:18 min

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September 08, 2021

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Marianna Vitiello², Claudia Kusmic, Francesco Faita, Laura Poliseno²

¹Institute of Clinical Physiology,National Research Council (IFC-CNR), ²Oncogenomics Unit,Core Research Laboratory, ISPRO

Chapters

00:04Introduction
01:04Melanoma Induction
01:58Imaging Procedure
04:18Post-Processing of Ultrasound Images
05:55Tissue-Specific and Inducible Braf/Pten Metastatic Melanoma Mouse Model
07:29Conclusion

Summary

Automatic Translation

黑色素瘤是一种非常具有侵袭性的疾病，可迅速扩散到其他器官。该协议描述了超高频超声成像的应用，结合3D渲染，以监测转移性黑色素瘤Braf / Pten小鼠模型中腹股沟淋巴结的体积。

Tags

Keywords: Lymph Node Volume Ultra-high-frequency Ultrasound Imaging Braf/Pten Genetically Engineered Mouse Model Melanoma Metastatic Disease Preclinical Mouse Models 4-hydroxytamoxifen Primary Tumor Subcutaneous Metastases Inguinal Lymph Nodes Histological Studies Anesthesia Physiological Parameters Depilatory Agent Acoustic Coupling Medium Linear Probe Region-of-interest Scan Distance Step Size

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