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通过真空辅助吸附剂提取从人类相关样品中捕获活性产生的微生物挥发性有机化合物
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Biochemistry
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JoVE Journal Biochemistry
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
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通过真空辅助吸附剂提取从人类相关样品中捕获活性产生的微生物挥发性有机化合物

DOI:
10.3791/62547-v

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09:19 min

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June 01, 2022

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, , , , , ,
1Department of Molecular Biology and Biochemistry,University of California Irvine, 2Department of Ecology and Evolutionary Biology,University of California Irvine, 3Entech Instruments Inc.

Chapters

  • 00:04Introduction
  • 00:59Stable Isotope Probing in Biological Samples Preparation
  • 02:17Sample Extraction
  • 04:10Analyze Samples on the Gas Chromatography Mass Spectrometer (GC-MS)
  • 04:56Data Analysis
  • 05:45Results: GC-MS, Heatmaps, and Data Analysis of the 13C-Labeled Volatiles in Fecal, Saliva, Sewage, Cultured and Uncultured Sputum Samples
  • 08:24Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了使用真空辅助吸附剂提取方法从生物样品中提取挥发性有机化合物，使用Entech样品制备导轨进行气相色谱结合质谱分析以及数据分析。它还描述了生物样品的培养和稳定同位素探测。

Tags

Microbial Volatile Organic CompoundsVASEVacuum-assisted Sorbent ExtractionVolatile MetabolitesBiomarker DiscoveryFecal SamplesSewage SamplesSaliva SamplesSputum SamplesVOA VialsCold Plate13C GlucoseDeuteriumBHI MediumTechnical Triplicates

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