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Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development
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Neuroscience
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JoVE Journal Neuroscience
Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development

Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development

DOI:
10.3791/62568-v

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04:20 min

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June 09, 2021

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, ,
1Department of Neurobiology,Duke University Medical Center

Chapters

  • 00:05Introduction
  • 00:39Preparing the Injection Syringe for Electroporation
  • 01:00Morphological Analyses of Cerebellar Granule Neurons (CGNs): 3D Reconstruction
  • 02:34Results: Analysis of Granule Neuron Morphology and Size During Cerebellar Development
  • 03:45Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a method to visualize synaptogenesis of granule neurons in the mouse cerebellum over the time course of postnatal brain development when these cells refine their synaptic structures and form synapses to integrate themselves into the overall brain circuit.

Tags

Cerebellar Granule NeuronsIn Vivo Postnatal ElectroporationNeurite MorphologyDendritic Claw FormationConfocal MicroscopySimple Neurite TracerFiji3D ReconstructionDevelopmental Processes

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