Journal
/
Biology
/
人类MK从人类主要来源的免疫细胞分拣或血细胞遗传基因 的体外 分化
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
Please note that all translations are automatically generated. Click here for the English version.

人类MK从人类主要来源的免疫细胞分拣或血细胞遗传基因 的体外 分化

DOI:
10.3791/62569-v

•

14:30 min

•

August 07, 2021

•

, , , , , , ,
1Platelet Research Lab,Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 2Flow Cytometry and Cell Sorting Platform (ISPA), 3Clinical Diagnosis Laboratory – Dept. of Hematology,Hospital Universitario Central de Asturias (HUCA), 4Department of Hematology,Hospital Universitario Severo Ochoa (HUSO), 5Department of Hematology,Instituto de Investigación Sanitaria San Carlos (IdISSC), Hospital Clínico San Carlos (HCSC), 6Dept. of Medicine,University of Oviedo

Chapters

  • 00:00Introduction
  • 01:17Background
  • 02:12Methods: Immunophenotyping
  • 03:50Results
  • 06:58Relevance
  • 07:42Methods: Cell Sorting
  • 12:27Results and Post-Sort Analysis
  • 12:53Limitations and Perspective

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了一个免疫嗜血策略，用于巨型胡萝卜细胞分化的特征，并展示该策略如何允许使用荧光激活细胞分拣器在不同阶段对巨型胡萝卜细胞进行排序。该方法可以应用于人体原生组织，也适用于 体外培养产生的巨型核细胞。

Tags

ImmunophenotypingCell SortingHuman MegakaryocytesHematopoietic ProgenitorsFlow CytometryMegakaryopoiesisPolyploidySurface MarkersMulti-omics StudiesPlatelet Production

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

人类胚胎干细胞：启动文化冷冻细胞

主电穿孔的小鼠骨髓造血细胞培养制备

从外植体生长的首要人权支气管上皮细胞

高效的推导人类心脏的前体和从多能干的小分子诱导人类胚胎干细胞的心肌细胞

表型分析和分离小鼠造血干细胞和传承的承诺祖

天真成人干细胞的分离从初级人类成纤维细胞培养

从小鼠和人类结肠组织初级肌成纤维细胞的分离

从人类体细胞与仙台病毒高效的新一代人类诱导多能干细胞

体外胰腺器官从分散的小鼠胚胎祖细胞

从人类肌肉提比利斯前部高级隔间收集骨骼肌肉活检，用于机械评估

Read Article