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Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane (SSM)-Based Electrophysiology
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Biochemistry
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JoVE Journal Biochemistry
Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane (SSM)-Based Electrophysiology

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane (SSM)-Based Electrophysiology

DOI:
10.3791/62578-v

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09:12 min

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May 03, 2021

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,
1Biozentrum, University of Basel

Chapters

  • 00:04Introduction
  • 00:58Measuring the Solute Transportation: Determination of Saturation Conditions
  • 03:16Serial Classification of Inhibitory and Non-Inhibitory Nanobodies
  • 05:59IC50 Measurement with Inhibitory Nanobodies
  • 07:40Results: Selection of Transporter Targeted Inhibitory Nanobodies by SSM Based Electrophysiology
  • 08:41Conclusion

Summary

Automatic Translation

Automatic Translation

Nanobodies are important tools in structural biology and pose a great potential for the development of therapies. However, the selection of nanobodies with inhibitory properties can be challenging. Here we demonstrate the use of solid-supported-membrane (SSM)-based electrophysiology for the classification of inhibitory and non-inhibitory nanobodies targeting electrogenic membrane transporters.

Tags

Keywords: Transporter-targeted Inhibitory NanobodiesSolid-supported-membrane (SSM)-based ElectrophysiologyHigh-throughput ScreeningElectrogenic TransportersProteoliposomesMembrane VesiclesNanobodiesMedical ApplicationsElectrogenic TransportersSSM BufferActivating SSM BuffersProteoliposome-coated ChipConductivityCapacitanceActivating SolutionsNonactivating BufferBAB SequenceProtein-free Liposomes

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