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הדמיה וכימות של TGFβ/BMP/SMAD איתות בתנאי לחץ שונים של גיסת נוזלים באמצעות קרבה-קשירה-אסאי
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Chapters
- 00:05Introduction
- 00:40Cell Culture and Fluid Shear Stress Exposure
- 02:55Fixation
- 04:58Blocking and Incubation with Primary Antibody
- 06:28Incubation with PLA Probe and Ligation
- 07:34Amplification and Mounting
- 09:14Results: Effect of Shear Stress on the SMAD Protein Interactions
- 11:02Conclusion
