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来自分离人产品的γ-Delta（γδ）T细胞的扩增和富集
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product
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来自分离人产品的γ-Delta（γδ）T细胞的扩增和富集

DOI:
10.3791/62622-v

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11:51 min

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September 22, 2021

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, , , , ,
1Cell Therapy Facility,Moffitt Cancer Center and Research Institute, 2Department of Immunology,Moffitt Cancer Center and Research Institute

Chapters

  • 00:04Introduction
  • 01:29Lymphocyte Isolation and Alpha-beta (αβ) T Cell Depletion
  • 06:16Co-culture with Artificial Antigen-Presenting Cells (aAPCs) and Cell Harvest
  • 09:22Results: Characterization and Optimization of the γδ T Cell Expansion Process
  • 11:21Conclusion

Summary

Automatic Translation

Automatic Translation

提出的是用于扩增γ-Δ（γδ）T细胞药物产物的方案。淋巴细胞通过洗脱分离，并富含唑来膦酸和白细胞介素-2的γδ。使用临床级磁分离装置消耗α-β-T细胞。γδ细胞与K562衍生的人工抗原呈递细胞共培养并扩增。

Tags

Keywords: Gamma-delta T CellsCytotoxic ActivityCancerAcute Myeloid LeukemiaExpansionEnrichmentApheresisCell TherapyElutriationCell CultureBioreactorZoledronic AcidIL-2Flow Cytometry

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