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OnePot PURE Cell-Free System

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

OnePot PURE Cell-Free System

OnePot PURE Cell-Free System

DOI:

10.3791/62625-v

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08:25 min

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June 23, 2021

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Laura Grasemann*¹, Barbora Lavickova*¹, M. Carolina Elizondo-Cantú, Sebastian J. Maerkl

¹Institute of Bioengineering, School of Engineering,École Polytechnique Fédérale de Lausanne

Chapters

00:04Introduction
00:54OnePot Protein Solution Expression and Purification
05:44OnePot PURE Reaction
06:51Results: OnePot Protein Purification and Overexpression
07:44Conclusion

Summary

Automatic Translation

We present a fast and cost-effective method to produce the recombinant PURE cell-free TX-TL system using standard laboratory equipment.

Tags

AI Writing Assistant Cell-free Protein Expression PURE System OnePot Method Recombinant Protein Purification Streamlined Protein Production Time-efficient System Cost-effective Platform Synthetic Cell-free Biology

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