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Journal
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Bioengineering
/
ワンポット純粋な無細胞システム
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
OnePot PURE Cell-Free System
Please note that all translations are automatically generated.
Click here for the English version.
ワンポット純粋な無細胞システム
DOI:
10.3791/62625-v
•
08:25 min
•
June 23, 2021
•
Laura Grasemann*
1
,
Barbora Lavickova*
1
,
M. Carolina Elizondo-Cantú
,
Sebastian J. Maerkl
1
Institute of Bioengineering, School of Engineering
,
École Polytechnique Fédérale de Lausanne
Chapters
00:04
Introduction
00:54
OnePot Protein Solution Expression and Purification
05:44
OnePot PURE Reaction
06:51
Results: OnePot Protein Purification and Overexpression
07:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
標準的な実験装置を用いて組換え純粋な無細胞TX-TLシステムを製造するための迅速かつ費用対効果の高い方法を提示します。
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Cell-free Protein Expression
PURE System
OnePot Method
Recombinant Protein Purification
Streamlined Protein Production
Time-efficient System
Cost-effective Platform
Synthetic Cell-free Biology
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