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Flowcytometrische analyse van apoptotische biomarkers in actinomycine D-behandelde SiHa baarmoederhalskankercellen
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
DOI:

13:53 min

August 26, 2021

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Chapters

  • 00:04Introduction
  • 00:43Preparation of Cell Culture and Treatments for Flow Cytometry
  • 02:56Enumerating Cells Using the Viability Assay
  • 03:36Staining Cells for Flow Cytometry
  • 08:00Run Samples on the Flow Cytometer
  • 10:04Post-Acquisition and Statistical Analysis
  • 11:20Results: Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
  • 13:01Conclusion

Summary

Automatic Translation

Apoptose kan worden gekenmerkt door flowcytometrische analyse van vroege en late apoptotische biomarkers. De cervicale kankercellijn, SiHa, werd geanalyseerd op apoptosebiomarkers na behandeling met Actinomycine D met behulp van een benchtop flowcytometer.

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