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Biochemistry

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JoVE Journal Biochemistry
Isolering af aktiv caenorhabditis elegans nukleare ekstrakt og rekonstitution for In Vitro Transskription
 
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Isolering af aktiv caenorhabditis elegans nukleare ekstrakt og rekonstitution for In Vitro Transskription

Article DOI: 10.3791/62723-v • 06:09 min • August 11th, 2021
August 11th, 2021 •
1, 1
1Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine, Washington State University

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Her beskriver vi en detaljeret protokol til isolering af aktivt nukleart ekstrakt fra larvestadiet 4 C. elegans og visualisering af transskriptionsaktivitet i et in vitro-system .

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Biokemi Udgave 174 C. elegans nukleart ekstrakt in vitro transskription Balch homogenisator ikke-radioaktiv påvisning
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