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Immunology and Infection

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短发夹RNA介导的基因敲低在造血干和祖细胞系体外发育的树突状细胞发育研究

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Immunology and Infection

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JoVE Journal Immunology and Infection

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Please note that all translations are automatically generated. Click here for the English version.

短发夹RNA介导的基因敲低在造血干和祖细胞系体外发育的树突状细胞发育研究

DOI:

10.3791/62730-v

•

06:12 min

•

March 07, 2022

•

Yu-Ling Hsiao, Hans Häcker, Chien-Kuo Lee

¹Graduate Institute of Immunology,National Taiwan University College of Medicine, ²Department of Pathology, Division of Microbiology and Immunology,University of Utah

Chapters

00:04Introduction
00:43Preparation of Immortalized HematopoieticStem and Progenitor Cell (iHSPC) Lines and Lentiviral Transduction
02:08In Vitro Differentiation of the Stable Knockdown iHSPC Cell Lines
03:05Flow Cytometric Analysis of the Differentiated Dendritic Cells
04:01Results: Dendritic Cell Differentiation Following Short Hairpin RNA-Mediated Tcf4 and Id2 Knockdown
05:33Conclusion

Summary

Automatic Translation

在这里，我们提供了一种方案，用于筛选参与树突状细胞（DC）发展的潜在转录因子，使用shRNA的慢病毒转导来获得稳定的敲低细胞系，用于体外 DC分化。

Tags

Keywords: Dendritic Cell Development Short Hairpin RNA Gene Knockdown Hematopoietic Stem And Progenitor Cell Line In Vitro Transcription Factors FLT3 Ligand Beta Estradiol Lentiviral Transduction Puromycin LacZ Tcf4 Id2 Flow Cytometry

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