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Undersøgelse af dendritisk celleudvikling af short hairpin RNA-medieret gen knockdown i en hæmatopoietic stilk og stamfader Cell Line In vitro
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Immunology and Infection
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JoVE Journal Immunology and Infection
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Undersøgelse af dendritisk celleudvikling af short hairpin RNA-medieret gen knockdown i en hæmatopoietic stilk og stamfader Cell Line In vitro

DOI:
10.3791/62730-v

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06:12 min

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March 07, 2022

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, ,
1Graduate Institute of Immunology,National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology,University of Utah

Chapters

  • 00:04Introduction
  • 00:43Preparation of Immortalized HematopoieticStem and Progenitor Cell (iHSPC) Lines and Lentiviral Transduction
  • 02:08In Vitro Differentiation of the Stable Knockdown iHSPC Cell Lines
  • 03:05Flow Cytometric Analysis of the Differentiated Dendritic Cells
  • 04:01Results: Dendritic Cell Differentiation Following Short Hairpin RNA-Mediated Tcf4 and Id2 Knockdown
  • 05:33Conclusion

Summary

Automatic Translation

Automatic Translation

Her leverer vi en protokol til screening af potentielle transskriptionsfaktorer, der er involveret i udviklingen af dendritiske celle (DC) ved hjælp af lentiviral transduktion af shRNA for at opnå stabile knockdown cellelinjer til in vitro DC differentiering.

Tags

Dendritic Cell DevelopmentShort Hairpin RNAGene KnockdownHematopoietic Stem And Progenitor Cell LineIn VitroTranscription FactorsFLT3 LigandBeta EstradiolLentiviral TransductionPuromycinLacZTcf4Id2Flow Cytometry

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