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微电极阵列记录中天节点发射率,以识别小鼠内在心脏起搏缺陷
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Medicine
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JoVE Journal
Medicine
Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice
Please note that all translations are automatically generated.
Click here for the English version.
微电极阵列记录中天节点发射率,以识别小鼠内在心脏起搏缺陷
DOI:
10.3791/62735-v
•
09:20 min
•
July 05, 2021
•
Praveen Kumar
,
Man Si
,
Kelsey Paulhus
,
Edward Glasscock
1
Department of Biological Sciences
,
Southern Methodist University
Chapters
00:04
Introduction
00:58
Dissecting the Sinoatrial Node
03:50
Microelectrode Array System Preparation for Recording
04:18
Heart Tissue Placement on Microelectrode Array Grid
05:03
Data Acquisition
06:10
Analysis of Microelectrode Array Recordings for Sinoatrial Node Beat Frequency Measurement
07:13
Results: The Sinoatrial Node Beat Frequency Measurement
08:41
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议旨在描述一种新的方法,测量内在心脏发射率使用微电极阵列记录整个中天节点组织,以确定小鼠的制动缺陷。药理制剂也可以在这种方法中引入,以研究它们对内在起搏的影响。
Tags
Microelectrode Array
Sinoatrial Node
Heart Rate
Cardiac Pacemaking
Mouse
Dissection
Tyrode’s Solution
Heart Removal
Atrium
Sinoatrial Node Location
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