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Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions
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JoVE Journal Cancer Research
Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions

Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions

DOI:
10.3791/62748-v

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07:39 min

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November 17, 2021

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, , , , , , , , , , , , , ,
1Team Tumoral signaling and therapeutic targets,UMR CNRS 7021 – Laboratory of Bioimaging and Pathologies, 2NETRI, 3Centre de Ressources Biologiques, Pathology department,University Hospital of Strasbourg, 4Pediatric Oncohematology unit,University Hospital of Strasbourg

Chapters

  • 00:04Introduction
  • 00:53Culturing in Microfluidic Device
  • 02:19Culturing Commercialized pHGG Cell Line: UW479 and BT35
  • 02:56Co-Culture Protocol
  • 03:59Electrophysiological Recording
  • 04:41Results: Co-culture of Glutamatergic Neurons
  • 06:55Conclusion

Summary

Automatic Translation

Automatic Translation

Recent works uncover the neuronal impact on high-grade pediatric glioma (pHGG) cells and their reciprocal interactions. The present work shows the development of an in vitro model co-culturing pHGG cells and glutamatergic neurons and recorded their electrophysiological interactions to mimic those interactivities.

Tags

Co-cultureGlutamatergic NeuronsPediatric High-grade Glioma CellsMicrofluidic DevicesElectrical InteractionsIPS-derived Cortical NeuronsUW479 CellsBT35 CellsViabilityImage Analysis

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