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Journal
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Cancer Research
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通过透明硅胶窗进行纵向活体成像
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Longitudinal Intravital Imaging Through Clear Silicone Windows
Please note that all translations are automatically generated.
Click here for the English version.
通过透明硅胶窗进行纵向活体成像
DOI:
10.3791/62757-v
•
07:44 min
•
January 05, 2022
•
Laura Maiorino*
1,2,3
,
Margaret Shevik*
1,4,5
,
José M. Adrover
,
Xiao Han
6
,
Elias Georgas
8
,
John Erby Wilkinson
,
Harrison Seidner
,
Leonie Foerschner
,
David A. Tuveson
,
Yi-Xian Qin
,
Mikala Egeblad
1
Cold Spring Harbor Laboratory
,
2
Cold Spring Harbor Laboratory School of Biological Sciences
,
3
Koch Institute for Integrative Cancer Research
,
Massachusetts Institute of Technology
,
4
Medical Scientist Training Program, School of Medicine
,
Stony Brook University
,
5
Graduate Program in Pharmacology
,
Stony Brook University
,
6
Graduate Program in Genetics
,
Stony Brook University
,
7
Graduate Program in Biomedical Engineering
,
Stony Brook University
,
8
Department of Biomedical Engineering
,
Stony Brook University
,
9
Department of Pathology
,
University of Michigan
Chapters
00:04
Introduction
00:50
Casting the Silicone Window and Preparing the Mouse for Insertion of the Silicone Window
01:44
Inserting a Lateral Window for Imaging in the Liver and the Pancreas
03:51
Inserting the Window for Imaging in the Mammary Gland
04:56
Imaging Through the Window
06:04
Results: Investigation of the Adaptability of the PDMS Window to Diverse Tissue Sites
07:05
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了一种使用光学透明硅胶窗口进行长期活体内成像的方法,该窗口可以直接粘附在感兴趣的组织/器官和皮肤上。这些窗户比目前在该领域使用的其他窗户更便宜,用途更广,手术插入对动物造成有限的炎症和痛苦。
Tags
Keywords: Intravital Imaging
Silicone Windows
PDMS
Longitudinal Imaging
Surgical Technique
Liver
Pancreas
Mammary Gland
Cancer
Immune Cells
Sterile Procedure
Surgical Adhesive
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