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通过透明硅胶窗进行纵向活体成像
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Longitudinal Intravital Imaging Through Clear Silicone Windows
DOI:

07:44 min

January 05, 2022

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Chapters

  • 00:04Introduction
  • 00:50Casting the Silicone Window and Preparing the Mouse for Insertion of the Silicone Window
  • 01:44Inserting a Lateral Window for Imaging in the Liver and the Pancreas
  • 03:51Inserting the Window for Imaging in the Mammary Gland
  • 04:56Imaging Through the Window
  • 06:04Results: Investigation of the Adaptability of the PDMS Window to Diverse Tissue Sites
  • 07:05Conclusion

Summary

Automatic Translation

本文介绍了一种使用光学透明硅胶窗口进行长期活体内成像的方法,该窗口可以直接粘附在感兴趣的组织/器官和皮肤上。这些窗户比目前在该领域使用的其他窗户更便宜,用途更广,手术插入对动物造成有限的炎症和痛苦。

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