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使用同质珠为基础的检测的查佩龙-科查佩龙相互作用研究
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay
DOI:

06:51 min

July 21, 2021

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Chapters

  • 00:04Introduction
  • 01:28Coupling of Hsp90 C-Terminal Peptide to the Acceptor Beads
  • 02:49Assay for Interaction Between GST-FKBP51 or GST-FKBP52 and Hsp90 C-Terminal Peptide and Inhibition with Small Molecular Mass Compounds
  • 04:14Dose-Response Curve Generation and IC50 Calculation
  • 04:58Results: Inhibitory Effect of Small Molecular Weight Test Compound D10
  • 05:59Conclusion

Summary

Automatic Translation

该协议提出了一种利用谷胱甘肽相关的供体珠与 GST 融合的 TPR-motif 共同伴奏和接受珠以及 Hsp90 衍生肽来探索蛋白质- 蛋白质相互作用的技术。我们使用此技术筛选小分子,以破坏 Hsp90-FKBP51 或 Hsp90-FKBP52 相互作用,并识别出有效和选择性的 Hsp90-FKBP51 相互作用抑制剂。

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