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JoVE Journal Cancer Research
Fluorescensmikroskopi för ATP-internalisering medierad av makropinocytos hos mänskliga tumörceller och tumör-xenograferade möss
 
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Fluorescensmikroskopi för ATP-internalisering medierad av makropinocytos hos mänskliga tumörceller och tumör-xenograferade möss

Article DOI: 10.3791/62768-v • 08:31 min • June 30th, 2021
June 30th, 2021 •
*1,2,3, *4, 1,5, 4, 1,2,4, 1,2,4, 6, 1,2,4,7
1Department of Biological Sciences, Ohio University, 2Molecular and Cellular Biology Program, Ohio University, 3Neuroscience Program, Ohio University, 4Edison Biotechnology Institute, Ohio University, 5Translational Biomedical Sciences Program, Ohio University, 6Honors Tutorial College, Ohio University, 7Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University
* These authors contributed equally

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Vi utvecklat en reproducerbar metod för att visualisera internalisering av nonhydrolyzable fluorescerande adenosin triphosphate (ATP), en ATP surrogat, med hög cellulär upplösning. Vi validerade vår metod med oberoende in vitro och in vivo analyser-mänskliga tumör cellinjer och immunodeficient möss xenografted med mänskliga tumör vävnad.

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Cancerforskning nummer 172
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