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用于 老德法纹 状体唾液采集的双层膜夹层法

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Biology

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JoVE Journal Biology

Two-layered Membrane Sandwich Method for Laodelphax striatellus Saliva Collection

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用于老德法纹状体唾液采集的双层膜夹层法

DOI:

10.3791/62831-v

•

06:13 min

•

August 27, 2021

•

Jing Zhao^2,3, Jie Yang^2,3, Lili Zhang², Rongxiang Fang², Yan Huo²

¹State Key Laboratory of Plant Genomics, Institute of Microbiology,Chinese Academy of Sciences, ²National Plant Gene Research Center, ³University of the Chinese Academy of Sciences

Chapters

00:05Introduction
01:02Preparation of Feeding Chamber and Artificial Diet
02:00Collection of Small Brown Planthopper (SBPH) Saliva
02:56Concentration of the Collected Saliva
03:58Results: Artificial Feeding and Saliva Collection from Laodelphax striatellus
05:21Conclusion

Summary

Automatic Translation

本方案描述了一种使用人工介质从穿刺-吸吮昆虫中收集足够唾液的方法。这是一种收集昆虫唾液和研究唾液对昆虫进食行为和媒介传播病毒传播的有用方法。

Tags

Keywords: Membrane Sandwich Method Laodelphax Striatellus Saliva Collection Artificial Feeding Saprophytic Insects Sucrose Solution Plant Hopper Larvae Feeding Chamber Paraffin Membrane Artificial Diet Aluminum Foil Sterile Tubes Centrifugal Filter

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