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测量CD95死亡诱导信号传导复合物的组成和该复合物中Procaspase-8的处理
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex
DOI:

07:17 min

August 02, 2021

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Chapters

  • 00:05Introduction
  • 00:44Preparing Cells and CD95L Stimulation
  • 01:11Cell Harvest and Lysis
  • 02:29Immunoprecipitation
  • 03:28Western Blot
  • 04:21Western Blot Detection
  • 05:05Analysis of CD95 DISC Formation in HeLa-CD95 cells, c-FLIPL-overexpressing HeLa-CD95 cells, and Primary T Cells
  • 06:25Conclusion

Summary

Automatic Translation

在这里,提出了一种实验工作流程,可以直接在死亡诱导信号复合物(DISC)上检测caspase-8处理并确定该复合物的组成。这种方法具有广泛的应用,从解开细胞死亡途径的分子机制到细胞凋亡网络的动态建模。

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