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En tredimensionel spheroid model til at undersøge tumor-stromal interaktion i hepatocellulær karcinom
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1Department of Biochemistry, Faculty of Pharmacy,Minia University, 2National Institute for Health Research Birmingham Liver Biomedical Research Unit and Centre for Liver and Gastrointestinal Research, Institute of Immunology and Immunotherapy,University of Birmingham, 3Newcastle Fibrosis Research Group, Biosciences Institute, Faculty of Medical Sciences,Newcastle University, 4Newcastle University Translational and Clinical Research Institute, Faculty of Medical Sciences,Newcastle University, 5The Liver Unit, Department of Medicine, Freeman Hospital,Newcastle-upon-Tyne Hospitals NHS Foundation Trust
Chapters
- 00:05Introduction
- 01:20Cell Preparation
- 02:17Cell Collection and Counting
- 04:14Collection of Fibroblast Conditioned Media
- 05:17Heterotypic Tumor/Stromal Spheroids
- 06:29Homotypic Hep3B Spheroids in LX2 Conditioned Media
- 08:18Calculation of Spheroid Volume
- 09:17Results: In Vitro Creation and Culture of 3D Tumor Spheroid Model
- 11:28Conclusion
Omfattende in vitro-modeller , der trofast generobrer den relevante menneskelige sygdom, mangler. Den nuværende undersøgelse præsenterer tre-dimensionelle (3D) tumor spheroid skabelse og kultur, en pålidelig in vitro værktøj til at studere tumor-stromal interaktion i human hepatocellulær karcinom.
