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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
 

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Article DOI: 10.3791/62878-v • 06:58 min • October 7th, 2021
October 7th, 2021 •
1, 1, 1
1Faculty of Biology, Technion - Israel Institute of Technology

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Summary
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Co-translational interactions play a crucial role in nascent-chain modifications, targeting, folding, and assembly pathways. Here, we describe Selective Ribosome Profiling, a method for in vivo, direct analysis of these interactions in the model eukaryote Saccharomyces cerevisiae.

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Keywords: Co-translational Interactions Selective Ribosome Profiling In Vivo Analysis Saccaromyces Cerevisiae Cryogenic Grinding RNase I Digestion Sucrose Cushion Affinity Purification Protein-ribosome Interactions Global Profiling
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