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1Molecular Microbial Pathogenesis Unit,Institut Pasteur, 2Chaire de Microbiologie et Maladies Infectieuses,Collège de France, 3Institut de Biologie Paris Seine (IBPS) – Developmental Biology Unit,Sorbonne Université, CNRS UMR7622, INSERM U1156, 4Molecular Radiotherapy, INSERM U1030, Gustave Roussy,Université Paris-Saclay, 5The Center for Microbes, Development and Health,Institut Pasteur Shanghai and Chinese Academy of Sciences, 6Microenvironment and Immunity Unit,Institut Pasteur
Chapters
- 00:05Introduction
- 01:07Intestinal Organoids Culture
- 04:13Visualization of Oxidative Stress in 3D Organoids by Confocal Microscopy
- 05:42Quantification of the Oxidative Stress on the Dissociated Organoids Using Flow Cytometer
- 07:19Results: Detection of ROS in the Intestinal Murine Organoids Using Qualitative Imaging and Quantitative Cytometry Assays
- 08:36Conclusion
The present protocol describes a method to detect reactive oxygen species (ROS) in the intestinal murine organoids using qualitative imaging and quantitative cytometry assays. This work can be potentially extended to other fluorescent probes to test the effect of selected compounds on ROS.
