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基于人类原发性皮肤成纤维细胞的亨廷顿舞蹈症模型微管加端动力学可视化
JoVE Journal
Bioengineering
Author Produced
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JoVE Journal Bioengineering
Microtubule Plus-End Dynamics Visualization in Huntington’s Disease Model based on Human Primary Skin Fibroblasts
DOI:

10:38 min

January 08, 2022

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Chapters

  • 00:05Introduction
  • 00:44Protocol
  • 01:02Obtaining a Primary Culture of Human Skin Fibroblasts
  • 02:14Cell Cultivation
  • 04:45Transfection
  • 06:46Preparing for Imaging
  • 08:08Setting the Imaging Parameters
  • 09:45Results
  • 10:13Conclusion

Summary

Automatic Translation

该协议专门用于通过EB3蛋白转染进行微管加端可视化,以研究它们在原代细胞培养物中的动态特性。该协议是在从亨廷顿舞蹈症患者获得的人类原发性皮肤成纤维细胞上实施的。

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