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Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness
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Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness

Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness

DOI:
10.3791/62973-v

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08:52 min

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March 18, 2022

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, , , , ,
1Harrington Laboratory for Molecular Orthopedics, Department of Orthopedic Surgery,University of Kansas Medical Center, 2Division of Sports Medicine, Department of Orthopedic Surgery,University of Kansas Medical Center, 3Department of Biochemistry & Molecular Biology,University of Kansas Medical Center

Chapters

  • 00:04Introduction
  • 00:50Creation of Knee OA in Mice
  • 01:04Tissue Preparation and Histologic Imaging
  • 01:35Quantitative Measurement of Osteoarthritic Subchondral Bone with ImageJ Software
  • 05:01Statistics
  • 05:25Representative Results
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

This methodology article presents a software-assisted quantitative measurement protocol to quantify histologic subchondral bone thickness in murine osteoarthritic knee joints and normal knee joints as controls. This protocol is highly sensitive to subtle thickening and is suitable for detecting early osteoarthritic subchondral bone changes.

Tags

Keywords: Subchondral Bone ThicknessOsteoarthritisQuantitative MeasurementImageJ SoftwareMouse ModelKnee OsteoarthritisArticular CartilageOsteophyte FormationSubchondral Bone ThickeningSoftware-assisted Quantification

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