Journal
/
/
To peeling metoder for isolering av photoreceptor celle rom i musen netthinnen for protein analyse
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis
DOI:

11:08 min

December 07, 2021

, ,

Chapters

  • 00:04Introduction
  • 00:52Live Cell Retinal Peeling Method
  • 03:35Lyophilized Retina Peeling Method
  • 07:42Results: Isolation of Subcellular Compartments of Murine Rod Photoreceptors for Protein Analysis
  • 10:23Conclusion

Summary

Automatic Translation

Denne protokollen presenterer to teknikker for å isolere de subcellulære rommene til murine rod fotoreseptorer for proteinanalyse. Den første metoden benytter levende netthinne og cellulosefilterpapir for å skille stang ytre segmenter, mens den andre bruker lyofilisert netthinne og tape for å skrelle bort stang indre og ytre segmentlag.

Related Videos

Read Article