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Journal
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Immunology and Infection
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使用晶体紫改善使用 TCID50 检测法进行病毒滴定的基于视觉细胞病变效应的读数
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays
Please note that all translations are automatically generated.
Click here for the English version.
使用晶体紫改善使用 TCID50 检测法进行病毒滴定的基于视觉细胞病变效应的读数
DOI:
10.3791/63063-v
•
04:06 min
•
February 12, 2022
•
Alba Frias-De-Diego
,
Elisa Crisci
1
College of Veterinary Medicine, Department of Population Health and Pathobiology
,
North Carolina State University
Chapters
00:02
Introduction
00:41
Titration Protocol
01:48
Staining Protocol
02:56
Results
03:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议显示了一种准确客观的方法，通过将晶体紫与光学显微镜和免疫细胞化学染色进行比较，可视化病毒滴定。
Tags
Viral Titration
TCID50 Assay
Crystal Violet Staining
Cytopathic Effect
Virus Stock
Cell Line
Serial Dilution
Viral Titer
Microscopy
Monocytochemistry Staining
Plaque-forming Unit Assay
Virology Research
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