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Culture and Imaging of Human Nasal Epithelial Organoids
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Culture and Imaging of Human Nasal Epithelial Organoids

Culture and Imaging of Human Nasal Epithelial Organoids

DOI:
10.3791/63064-v

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13:20 min

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December 17, 2021

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, , ,
1Gregory Fleming James Cystic Fibrosis Research Center,University of Alabama at Birmingham (UAB), 2Department of Pediatrics, Division of Pulmonary and Sleep Medicine,UAB

Chapters

  • 00:04Introduction
  • 01:40Cell Culture and Expansion of Nasal Epithelial Cells
  • 04:23Growth and Differentiation of Organoids in Slides and Culture Inserts
  • 06:06Isolation and Fixation of Organoids
  • 07:36Preparation and Isolation of Organoids for Histological Sectioning
  • 08:57Baseline Lumen Measurements
  • 09:56Results: Live and Fixed Imaging of the Airway Epithelial Organoids Derived from Nasal Epithelial Cells and Analysis Methods
  • 12:21Conclusion

Summary

Automatic Translation

Automatic Translation

A detailed protocol is presented here to describe an in vitro organoid model from human nasal epithelial cells. The protocol has options for measurements requiring standard laboratory equipment, with additional possibilities for specialized equipment and software.

Tags

Keywords: Nasal Epithelial OrganoidsCystic FibrosisCFTR ActivityEpithelial Ion TransportEpithelial Cell Fluid TransportPrimary Ciliary DyskinesiaCell DissociationCell Expansion3T3 FibroblastsCell CultureCell Imaging

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