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Chapters
- 00:04Introduction
- 01:40Cell Culture and Expansion of Nasal Epithelial Cells
- 04:23Growth and Differentiation of Organoids in Slides and Culture Inserts
- 06:06Isolation and Fixation of Organoids
- 07:36Preparation and Isolation of Organoids for Histological Sectioning
- 08:57Baseline Lumen Measurements
- 09:56Results: Live and Fixed Imaging of the Airway Epithelial Organoids Derived from Nasal Epithelial Cells and Analysis Methods
- 12:21Conclusion
Aquí se presenta un protocolo detallado para describir un modelo organoide in vitro de células epiteliales nasales humanas. El protocolo tiene opciones para mediciones que requieren equipos de laboratorio estándar, con posibilidades adicionales para equipos y software especializados.
