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Kultur och avbildning av humana nasala epitelorganoider
JoVE Journal
Medicine
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JoVE Journal Medicine
Culture and Imaging of Human Nasal Epithelial Organoids
DOI:

13:20 min

December 17, 2021

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Chapters

  • 00:04Introduction
  • 01:40Cell Culture and Expansion of Nasal Epithelial Cells
  • 04:23Growth and Differentiation of Organoids in Slides and Culture Inserts
  • 06:06Isolation and Fixation of Organoids
  • 07:36Preparation and Isolation of Organoids for Histological Sectioning
  • 08:57Baseline Lumen Measurements
  • 09:56Results: Live and Fixed Imaging of the Airway Epithelial Organoids Derived from Nasal Epithelial Cells and Analysis Methods
  • 12:21Conclusion

Summary

Automatic Translation

Ett detaljerat protokoll presenteras här för att beskriva en in vitro-organoidmodell från humana nasala epitelceller. Protokollet har alternativ för mätningar som kräver standard laboratorieutrustning, med ytterligare möjligheter till specialutrustning och programvara.

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