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Biology
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用于分析细胞信号的集成工具包:力、运动、形态学和荧光
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence
Please note that all translations are automatically generated.
Click here for the English version.
用于分析细胞信号的集成工具包:力、运动、形态学和荧光
DOI:
10.3791/63095-v
•
14:55 min
•
March 05, 2022
•
Alyson Nguyen*
1
,
Keith Battle*
2,3
,
Sunita S. Paudel*
2,3
,
Ningyong Xu
,
Jessica Bell
,
Linn Ayers
,
Cassandra Chapman
,
Ajay P. Singh
,
Srinivas Palanki
,
Thomas Rich
7
,
Diego F. Alvarez
,
Troy Stevens
3
,
Dhananjay T. Tambe
4,7
1
Biomedical Sciences, Pat Capps Covey College of Allied Health Professions
,
University of South Alabama
,
2
Department of Physiology and Cell Biology, College of Medicine
,
University of South Alabama
,
3
Center for Lung Biology, College of Medicine
,
University of South Alabama
,
4
William B. Burnsed Jr. Mechanical, Aerospace, and Biomedical Engineering Department, College of Engineering
,
University of South Alabama
,
5
Mitchell Cancer Institute, College of Medicine
,
University of South Alabama
,
6
Department of Chemical and Biomedical Engineering
,
West Virginia University
,
7
Department of Pharmacology, College of Medicine
,
University of South Alabama
,
8
Department of Physiology & Pharmacology, College of Osteopathic Medicine
,
Sam Houston State University
Chapters
00:02
Introduction
01:46
Creation of Position List
03:57
Automated Image Acquisition for the Remaining Experiment
04:34
Setting Up Automated Data Analysis
06:08
Quantification of Hydrogel Deformation
06:37
Quantification of Cell-ECM and Cell-cell Forces
08:44
Segmentation of the Individual Cells in the Images
09:47
Mapping of the Pixel Intensities on the Cells
11:06
Mapping of the Forces and Motion on the Cells
11:51
Tracking of the Cellular Indentities across the Experiment
12:51
Generation of Time Tracks of the Assessed Cellular Properties
13:27
Generation of the Heat Maps of the Assessed Cellular Properties
13:53
Representative Results
14:30
Conclusion
Summary
Automatic Translation
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Automatic Translation
用于分析细胞信号的集成工具包 (iTACS) 平台可自动测量贴壁细胞中的各种化学和机械信号。iTACS旨在促进社区驱动的开发,并使研究人员能够使用所有平台功能,无论其教育背景如何。
Tags
Cellular Signals
Forces
Motion
Morphology
Fluorescence
Monolayer Stress Microscopy
Hydrogel
Fluorescent Beads
AcTrM
AnViM
Cell Monolayer
Image Acquisition
Live View
Position List
Phase Imaging
Fluorescence Imaging
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