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Flow cytometrisk analyse af flere mitokondrieparametre i humaninducerede pluripotente stamceller og deres neurale og gliale derivater
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1Department of Clinical Medicine (K1),University of Bergen, 2Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases,Haukeland University Hospital, 3Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine,Shandong University, 4Shandong Key Laboratory of Brain Function Remodeling
Chapters
- 00:04Introduction
- 00:54Flow Cytometry Measurement of Mitochondrial Volume, MMP, and Mitochondrial ROS in Live Cells
- 02:26Flow Cytometry Measurement of MRC Complex Subunits and TFAM in Fixed Cells
- 03:49Flow Cytometry Analysis
- 04:14Results: Flow Cytometry Investigation of iPSC-Derived Neural Cells
- 05:07Conclusion
Denne undersøgelse rapporterer en ny tilgang til måling af flere mitokondrielle funktionelle parametre baseret på flowcytometri og dobbeltfarvning med to fluorescerende reportere eller antistoffer til påvisning af ændringer i mitokondrievolumen, mitokondriemembranpotentiale, reaktivt iltartsniveau, mitokondrie respiratorisk kædesammensætning og mitokondrie-DNA.
