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Flowcytometrisk analyse av multiple mitokondrielle parametere i humaninduserte pluripotente stamceller og deres nevrale og gliaderivater
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1Department of Clinical Medicine (K1),University of Bergen, 2Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases,Haukeland University Hospital, 3Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine,Shandong University, 4Shandong Key Laboratory of Brain Function Remodeling
Chapters
- 00:04Introduction
- 00:54Flow Cytometry Measurement of Mitochondrial Volume, MMP, and Mitochondrial ROS in Live Cells
- 02:26Flow Cytometry Measurement of MRC Complex Subunits and TFAM in Fixed Cells
- 03:49Flow Cytometry Analysis
- 04:14Results: Flow Cytometry Investigation of iPSC-Derived Neural Cells
- 05:07Conclusion
Denne studien rapporterer en ny tilnærming til å måle flere mitokondrielle funksjonelle parametere basert på flowcytometri og dobbeltfarging med to fluorescerende reportere eller antistoffer for å oppdage endringer i mitokondrielt volum, mitokondrielt membranpotensial, reaktivt oksygenartsnivå, mitokondriell respiratorisk kjedesammensetning og mitokondrielt DNA.
