Journal
/
Biology
/
用组织培养幼苗制备和饲养轴性昆虫用于叶甲虫的宿主 - 肠道微生物群相互作用研究
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Preparing and Rearing Axenic Insects with Tissue Cultured Seedlings for Host-Gut Microbiota Interaction Studies of the Leaf Beetle
Please note that all translations are automatically generated. Click here for the English version.

用组织培养幼苗制备和饲养轴性昆虫用于叶甲虫的宿主 - 肠道微生物群相互作用研究

DOI:
10.3791/63195-v

•

06:56 min

•

October 08, 2021

•

, , , ,
1State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences,Hubei University, 2Institute of Plant Protection,Wuhan Institute of Landscape Architecture, 3McKetta Department of Chemical Engineering,University of Texas at Austin

Chapters

  • 00:05Introduction
  • 00:54Insect Rearing
  • 01:43Germ-Free Poplar Culturing
  • 02:40Egg Surface Sterilization and Rearing Axenic Larvae
  • 03:47Verification of Axenic Larvae with Culture-Dependent Assays
  • 04:40Verification of Axenic Individuals with Culture-Independent Assays
  • 05:32Results: Confirmation of Efficacy of Gut Bacteria Elimination
  • 06:07Conclusion

Summary

Automatic Translation

Automatic Translation

为了获得轴性昆虫，其卵表面被灭菌，然后使用轴性叶子饲养孵化的幼虫。这种方法为抗焦虑昆虫的制备提供了一种有效的方法，而无需施用抗生素或开发人造饮食，这也可以应用于其他食叶昆虫。

Tags

Axenic InsectsGut MicrobiotaLeaf BeetlePlant SeedlingsAlpha-naphthalene Acetic AcidMS MediumSurface SterilizationGerm-freeArtificial DietInsect Rearing

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

适配器，网格蛋白相互作用在体内和体外研究

指南现代荧光定量免疫印迹与故障排除策略

从白蛉提取内分泌物的方法<em> Bemisia tabaci</em

应用被动头部运动在啮齿动物头部产生定义的加速度

巴西寄生体加纳 斯菌的饲养方法，这是一种有前途的生物控制剂，用于侵入性 铃木果蝇

培养的人间充质干细胞细胞外囊泡的分离、表征和治疗应用

模型线虫秀丽隐杆线虫天然微生物群的分离和表征

用于荧光和共聚焦成像的玉米叶原基横向切片和展开整片的制备方法的改进方法

优化无菌黄蜂的饲养程序

用半发酵无菌日粮饲养 Axenic Delia antiqua

Read Article