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High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model
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High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model

High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model

DOI:
10.3791/63284-v

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08:16 min

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March 16, 2022

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, , , , , , , ,
1Dewitt Daughtry Family Department of Surgery,University of Miami Miller School of Medicine, 2Vascular Biology Institute,University of Miami Miller School of Medicine, 3University of Miami, 4Analytical Imaging Core Facility,University of Miami

Chapters

  • 00:04Introduction
  • 01:19Equipment Setup and DiI Perfusion
  • 04:08Preparation of Footpad Tissue for Confocal Laser Scanning Microscopy
  • 05:01Confocal Laser Scanning Microscopy
  • 06:37Results: Anatomy of the Murine Hindlimb Vasculature
  • 07:20Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes a unique, clinically relevant model of peripheral arterial disease that combines femoral artery and vein electrocoagulation with the administration of a nitric oxide synthase inhibitor to induce hindlimb gangrene in FVB mice. Intracardiac DiI perfusion is then used for high-resolution, three-dimensional imaging of the footpad vasculature.

Tags

Keywords: 3D ImagingFoot PadVasculatureMurine HindlimbGangrene ModelDiI PerfusionNeovascularizationPeripheral Arterial DiseaseCritical Limb IschemiaPreclinical Research

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