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Journal
/
Biochemistry
/
トリパノソーマ・クルジ
または他の病原性生物由来のDNAを検出するためのすぐに使用できるqPCR
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Ready-To-Use qPCR for Detection of DNA from
Trypanosoma cruzi
or Other Pathogenic Organisms
Please note that all translations are automatically generated.
Click here for the English version.
トリパノソーマ・クルジ
または他の病原性生物由来のDNAを検出するためのすぐに使用できるqPCR
DOI:
10.3791/63316-v
•
05:50 min
•
January 20, 2022
•
Alexandre Dias Tavares Costa
2,3
,
Steffanie Skau Amadei
3
,
Amanda Bertão-Santos
2
,
Tuany Rodrigues
3
1
Laboratório de Ciências e Tecnologias Aplicadas em Saúde (LaCTAS), Instituto Carlos Chagas (ICC)
,
Fundação Oswaldo Cruz (Fiocruz)
,
2
Pós-graduação em Engenharia de Bioprocessos e Biotecnologia
,
Universidade Federal do Paraná (UFPR)
,
3
Pós-graduação em Biociências e Biotecnologia, Instituto Carlos Chagas (ICC)
,
Fundação Oswaldo Cruz (Fiocruz)
Chapters
00:04
Introduction
00:47
Preparation of Stock Solutions and Gelification Mixture
02:53
Preparation of qPCR Master Mix, Gelification of the Reagents in the Reaction Vessels, and Gelified qPCR
04:22
Results: Ready to Use Gelified qPCR for
Trypanosoma cruzi
Epimastigotes DNA Detection
05:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究では、反応容器にプリロードして冷蔵庫に数ヶ月間保存できる
、T. cruzi
DNA検出用のすぐに使用できるqPCRを製造するための手順について説明します。
Tags
QPCR
Trypanosoma Cruzi
Pathogenic Organisms
Gelification Protocol
Ready-to-use QPCR
Quality Control
Melezitose
Trehalose
Glycogen
Lysine
Stock Solutions
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