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使用3D结构照明显微镜（3D-SIM）测量轴突初始段的膜周期性骨架的性质

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)

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使用3D结构照明显微镜（3D-SIM）测量轴突初始段的膜周期性骨架的性质

DOI:

10.3791/63327-v

•

07:40 min

•

February 11, 2022

•

David Micinski, Lauri Lahti, Amr Abouelezz^3,4

¹Minerva Foundation Institute for Medical Research, ²Department of Computer Science,Aalto University School of Science, ³HiLIFE – Neuroscience Center,University of Helsinki, ⁴HiLIFE – Institute of Biotechnology,University of Helsinki

Chapters

00:04Introduction
00:49Sample Preparation
02:57Imaging
04:32Image Analysis
06:06Results: 3D-Structured Illumination Microscopy Image Analysis
06:51Conclusion

Summary

Automatic Translation

本方案描述了一种使用培养的大鼠海马神经元和3D结构照明显微镜（3D-SIM）可视化和测量肌动蛋白环和轴突初始节段的膜周期性骨架的其它组分的方法。

Tags

3D-SIM Membrane Periodic Skeleton Axon Initial Segment Fluorescence Microscopy Sample Preparation Ankyrin G Fluoro 4 Ferodin Mounting Immersion Oil

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