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2-fotonenmicroscopie gebruiken om de effecten van chronische unilaterale ureterale obstructie op glomerulaire processen te kwantificeren
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
DOI:

11:47 min

March 04, 2022

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Chapters

  • 00:05Introduction
  • 00:55Preparing the Munich Wistar Frmter or SD Rat for Unilateral Ureteral Obstruction (UUO) Surgery
  • 01:53Surgical Preparation for Intravital 2-Photon Imaging
  • 04:04Positioning the Rat for Imaging and Image Acquisition for Quantitative Analysis
  • 06:16Calculating Red Blood Cell Flow in Surface Glomerular Capillary Loops and Renal Vasculature Using a Linescan Function
  • 07:45Calculating White Blood Cell (WBC) Occlusion in Glomerular Capillary Loops
  • 08:38Results: Quantification of Glomerular Processes in the Unilateral Ureteral Obstruction (UUO) Model of Rats
  • 10:56Conclusion

Summary

Automatic Translation

Hier presenteren we een protocol met behulp van 2-fotonmicroscopie in München Wistar Fromter-ratten met oppervlakteglomeruli om de effecten van langdurige ureterale obstructie op glomerulaire dynamiek en functie te kwantificeren.

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