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Modified Technique for the Use of Neonatal Murine Hearts in the Langendorff Preparation
 

Modified Technique for the Use of Neonatal Murine Hearts in the Langendorff Preparation

Article DOI: 10.3791/63349-v • 08:23 min • March 4th, 2022
March 4th, 2022 •
1, 2
1Department of Anesthesiology, Vanderbilt University Medical Center, 2Department of Anesthesiology, Columbia University Medical Center

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Summary
Automatic Translation

The present protocol describes aortic cannulation and retrograde perfusion of the ex-vivo neonatal murine heart. A two-person strategy, using a dissecting microscope and a blunted small gauge needle, permits reliable cannulation. Quantification of longitudinal contractile tension is achieved using a force transducer connected to the apex of the left ventricle.

Tags

Keywords: Neonatal Murine Langendorff Preparation Retrograde Perfusion Cardiac Development Cardiomyocyte Isolation Aortic Cannula Krebs-Heneseleit Buffer Heparin Anesthetic Dissection
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