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Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
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JoVE Journal Medicine
Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

DOI:
10.3791/63352-v

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08:48 min

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February 16, 2022

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, , , , , ,
1Department of Anatomy & Cell Biology,Rush University Medical Center, 2Department of Microbial Pathogens and Immunity,Rush University Medical Center, 3Department of Pathology,Rush University Medical Center

Chapters

  • 00:04Introduction
  • 00:52Preparation of Antigen-Conjugated Microspheres
  • 03:24Assay Performance and Optimization of Specimen-Capture Incubation Time
  • 06:31Results: Monitoring of Dynamic Changes in Antibody Titers Using Multianalyte Covid-19 Immune Response Panel
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a convenient method for monitoring dynamic changes in antibody titers for two immunoglobulin isotypes concurrently (either IgA, IgM, or IgG) resulting from an immune response to SARS-CoV-2 infection or vaccination. This 'Multianalyte Covid-19 Immune Response Panel' employs three indirect immunoassays built on coded microspheres that are read using a flow-based multiplex reader with 'dual-channel' capability.

Tags

Keywords: SeroconversionMultianalyte Immunobead AssayCOVID-19Antibody DetectionHigh-throughputEpitope EvaluationCell SignalingImmune Response MonitoringMagnetic MicrospheresBead ActivationProtein CouplingMES BufferEDCNHS

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