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Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes
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Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes
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Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes

DOI:
10.3791/63378-v

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08:45 min

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May 10, 2022

•

,
1Center for Iron Disorders, David Geffen School of Medicine,University of California, Los Angeles

Chapters

  • 00:04Introduction
  • 00:37Preparation of 58Fe‐Transferrin
  • 03:37Administration of <sup58Fe‐Transferrin Intravenously to E17.5 Pregnant Mice
  • 05:53Tissue Processing for Quantitative Iron Analysis by Inductively Coupled Plasma Mass Spectrometry (ICP‐MS)
  • 07:14Results: Physiological Regulation of Mouse Placental Ferroportin During Maternal Iron Deficiency
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

This article demonstrates how to prepare and administer transferrin-bound nonradioactive isotopic iron for studies of iron transport in mouse pregnancy. The approach for quantifying isotopic iron in fetoplacental compartments is also described.

Tags

Keywords: Iron TransportMouse PlacentaNonradioactive Iron IsotopesStable Iron IsotopesIron UptakeIron RegulationIron DistributionFerric ChlorideFerric NitrilotriacetateApotransferrinTransferrin LoadingMolecular Weight Cutoff Column

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