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用于体外评估药物诱导肝毒性的人肝脏微生理系统

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro

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用于体外评估药物诱导肝毒性的人肝脏微生理系统

DOI:

10.3791/63389-v

•

11:06 min

•

January 31, 2022

•

Ovidiu Novac, Raul Silva, Lucy-May Young, Kim Lachani, David Hughes, Tomasz Kostrzewski

¹CN Bio Innovations Ltd

Chapters

00:05Introduction
00:53Microphysiological System (MPS) Set-Up and Priming (Day -1)
02:14Seeding Liver Cells into MPS on Day 0
06:00Media Collection, Media Change and Drug Dosing (Days 4 and 6), and Ending the Experiment (Day 8)
07:52Results: Assessment of Liver Microtissues After the Treatment with Various Drugs
10:22Conclusion

Summary

Automatic Translation

药物性肝损伤（DILI）是药物失败的主要原因。已经开发了一种协议来使用肝脏微生理系统（MPS）准确预测化合物的DILI责任。肝脏模型使用原代肝细胞的共培养和翻译相关终点来评估细胞对治疗的反应。

Tags

Human Liver Microphysiological System Drug-induced Liver Toxicity In Vitro 3D Culture Primary Human Hepatocytes Human Kupffer Cells Cell Culture Preclinical Safety Testing Drug Development

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