Journal
/
Biology
/
Isolering af dyrkbare gær og skimmelsvampe fra jord for at undersøge svampepopulationsstruktur
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Isolering af dyrkbare gær og skimmelsvampe fra jord for at undersøge svampepopulationsstruktur

DOI:
10.3791/63396-v

•

10:33 min

•

May 27, 2022

•

, ,
1Department of Biology,McMaster University

Chapters

  • 00:04Introduction
  • 00:50Incubation of Soil in Liquid Broth and Transferring the Supernatant to the Solid Medium
  • 02:34Detection of Yeasts, Streaking for Single Colonies, and Identification of Yeast Species
  • 04:04Isolation of Aspergillus fumigatus from Soil
  • 07:32Results: Isolation and Characterization of Soil Yeast and Aspergillus fumigatus Populations
  • 09:37Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol er en effektiv, hurtig metode til dyrkning af gær og formen Aspergillus fumigatus fra store sæt jordprøver på så lidt som 7 dage. Metoderne kan let ændres for at imødekomme en række inkubationsmedier og temperaturer efter behov for eksperimenter.

Tags

Soil SamplesYeast DiversityMold DiversityFungal PopulationCulturable YeastsCulturable MoldsChloramphenicolBenomylYeast Extract-peptone-dextrose AgarRoller Drum IncubationBiosafety CabinetSoil Particle SuspensionColony SelectionStreak Plating

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63396?article_type=v

Isolation of Genomic DNA from Mouse Tails
check_url/63396?article_type=v

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
check_url/63396?article_type=v

Isolering og oprensning af kinesin fra Drosophila Embryoner
check_url/63396?article_type=v

Isolation of Immune Cells from Primary Tumors
check_url/63396?article_type=v

Isolering af Small noncoding RNA'er fra human serum
check_url/63396?article_type=v

Isolering af myofibroblaster fra mus og human Spiserøret
check_url/63396?article_type=v

Analyse af Autophagy i<em> Penicillium chrysogenum</em> Ved hjælp Sult Pads i kombination med Fluorescence Microscopy
check_url/63396?article_type=v

Fjernelse af Eksogene Materialer fra den ydre del af frosne kerner at undersøge den gamle biologiske samfund nærede Inside
check_url/63396?article_type=v

Isolering og karakterisering af mikrovesikler fra perifert blod
check_url/63396?article_type=v

Teknikker til at fremkalde og kvantificere cellesenescens

Read Article