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Neuroscience
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快速高尔基染色用于海马体和前额叶皮层树突状脊柱可视化
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Rapid Golgi Stain for Dendritic Spine Visualization in Hippocampus and Prefrontal Cortex
Please note that all translations are automatically generated.
Click here for the English version.
快速高尔基染色用于海马体和前额叶皮层树突状脊柱可视化
DOI:
10.3791/63404-v
•
04:58 min
•
December 03, 2021
•
Maya Frankfurt
2
,
Rachel Bowman
1
Department of Science Education
,
Donald and Barbara Zucker School of Medicine at Hofstra/Northwell
,
2
Department of Psychology
,
Sacred Heart University
Chapters
00:04
Introduction
00:48
Sectioning of Brain Tissues and Mounting
02:04
Staining and Dehydration of Brain Tissue
03:06
Determination of Dendritic Spine Density
03:48
Results: Observation of Hippocampus and Effect of Environmental Enrichment on CA1 and mPFC
04:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了快速高尔基体方法的修改,该方法可以适应神经系统的任何部分,用于染色大鼠海马体和内侧前额叶皮层中的神经元。
Tags
Golgi Stain
Dendritic Spine
Hippocampus
Prefrontal Cortex
Cryostat Sectioning
Rapid Labeling
Golgi Impregnation
Neuron Analysis
Dendritic Length
Spine Density
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