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Genanvendelig enkeltcelle til iterative epigenomiske analyser
JoVE Journal
Genetics
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JoVE Journal Genetics
Reusable Single Cell for Iterative Epigenomic Analyses
DOI:

10:28 min

February 11, 2022

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Chapters

  • 00:05Introduction
  • 01:32Preparation of Reusable Single Cells: Manual Version
  • 03:09Random Primer Annealing and Extension
  • 04:09Antibody Binding and Proximity Ligation of Antibody Probe and Proximally Extended Random Primer
  • 05:25Full Extension of the 1st Random Primer and Multiple Displacement Amplification
  • 07:32Results: Single Cell Multiepigenomic Analysis
  • 09:26Conclusion

Summary

Automatic Translation

Denne protokol beskriver en enkeltcellemetode til iterative epigenomiske analyser ved hjælp af en genanvendelig enkeltcelle. Den genanvendelige enkeltcelle muliggør analyser af flere epigenetiske mærker i samme enkeltcelle og statistisk validering af resultaterne.

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