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In Ovo Intravascular Injection in Chicken Embryos
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Developmental Biology
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JoVE Journal Developmental Biology
In Ovo Intravascular Injection in Chicken Embryos

In Ovo Intravascular Injection in Chicken Embryos

DOI:
10.3791/63458-v

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07:00 min

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June 03, 2022

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, , , , , , , , , , , , ,
1Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China,Yangzhou University, 2Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology,Yangzhou University, 3Animal & Avian Sciences,University of Maryland, College Park, 4College of Biotechnology,Jiangsu University of Science and Technology

Chapters

  • 00:10Introduction
  • 01:33Fertilized Egg Collection and Preparation
  • 02:39Preparation Before Injection
  • 03:07Windowing
  • 03:36Injection
  • 05:43Representative Results
  • 06:31Conclusion

Summary

Automatic Translation

Automatic Translation

The overall goal of this paper is to describe how to perform in ovo intracellular injection of exogenous materials into chicken embryos. This approach is very useful to study the developmental biology of chicken embryos.

Tags

Keywords: Chicken EmbryoDevelopmental ResearchIn Ovo InjectionVascular InjectionFertilized EggIncubationNeedle PreparationWindowingPlasmidLentivirusPrimordial Germ CellsTrypan Blue

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