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小鼠脑实质小动脉内皮的分离及功能分析

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Biology

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JoVE Journal Biology

Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma

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小鼠脑实质小动脉内皮的分离及功能分析

DOI:

10.3791/63463-v

•

06:40 min

•

March 11, 2022

•

Md A. Hakim, Paulo W. Pires, Erik J. Behringer

¹Department of Basic Sciences,Loma Linda University, ²Departments of Physiology, Surgery and Neurosurgery and Sarver Heart Center,University of Arizona College of Medicine Tucson

Chapters

00:04Introduction
00:58Dissection and Isolation of Mouse Cerebral Arterioles
02:30Digestion of Parenchymal Arterioles and Preparation of Endothelial Tubes
03:47Utilization of Arteriolar Endothelial Tubes for the Examination of Cellular Physiology and Cellular Imaging
04:54Results: Assessment of Endothelial Function
05:50Conclusion

Summary

Automatic Translation

来自脑实质小动脉的完整小鼠脑内皮“管”的强化制备用于研究脑血流调节。此外，我们证明了这种内皮研究模型在荧光成像和关键细胞信号通路的电生理学测量方面的实验优势，包括细胞内[Ca^{2 +}]和膜电位的变化。

Tags

Arteriolar Endothelium Parenchymal Arterioles Intracerebral Blood Flow Neuronal And Glial Activity Morphological Dimensions Calcium And Electrical Signaling Endothelial Cells Enzymatic Digestion Dissociation Solution Papain Dithioerythritol Collagenase Elastase Arteriolar Segments Endothelial Tubes

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