Journal
/
Medicine
/
Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

DOI:
10.3791/63469-v

•

05:08 min

•

January 31, 2022

•

,
1i3S – Instituto de Investigação e Inovação em Saúde,Universidade do Porto, 2Basic and Clinical Research on Iron Biology, IBMC – Instituto de Biologia Molecular e Celular,Universidade do Porto, 3Iron and Innate Immunity, IBMC – Instituto de Biologia Molecular e Celular,Universidade do Porto, 4ICBAS – Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto

Chapters

  • 00:04Introduction
  • 00:49Sample Drying
  • 01:33Sample Acidic Digestion
  • 02:11Color Development and Absorbance Reading
  • 02:36Results: Cuvette Versus 96-Well Microplate Comparison, Measurement of Non-Heme Iron Levels in a Mouse Model of Genetic Hemochromatosis, in Sea Bass Liver, and Whole Drosophila melanogaster
  • 04:41Conclusion

Summary

Automatic Translation

Automatic Translation

Here, a protocol for the measurement of the non-heme iron content in animal tissues is provided, using a simple, well-established colorimetric assay that can be easily implemented in most laboratories.

Tags

Bathophenanthroline-based Colorimetric AssayMicroplate FormatIron OverloadIron DeficiencyAcid ExtractionChromogen ReactionSpectrophotometric Measurement

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63469?article_type=v

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
check_url/63469?article_type=v

Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography
check_url/63469?article_type=v

Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer
check_url/63469?article_type=v

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation
check_url/63469?article_type=v

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform
check_url/63469?article_type=v

Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure
check_url/63469?article_type=v

Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model
check_url/63469?article_type=v

Segmentation and Linear Measurement for Body Composition Analysis using Slice-O-Matic and Horos
check_url/63469?article_type=v

Measurement of Tissue Oxygenation Using Near-Infrared Spectroscopy in Patients Undergoing Hemodialysis
check_url/63469?article_type=v

Human Subcutaneous Adipose Tissue Sampling Using a Mini-Liposuction Technique

Read Article