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使用基于菲咯啉的比色法测量组织非血红素铁含量
JoVE Journal
Medicine
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JoVE Journal Medicine
Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
DOI:

05:08 min

January 31, 2022

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Chapters

  • 00:04Introduction
  • 00:49Sample Drying
  • 01:33Sample Acidic Digestion
  • 02:11Color Development and Absorbance Reading
  • 02:36Results: Cuvette Versus 96-Well Microplate Comparison, Measurement of Non-Heme Iron Levels in a Mouse Model of Genetic Hemochromatosis, in Sea Bass Liver, and Whole Drosophila melanogaster
  • 04:41Conclusion

Summary

Automatic Translation

在这里,提供了一种测量动物组织中非血红素铁含量的方案,使用一种简单,成熟的比色法,可以在大多数实验室中轻松实施。

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