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使用基于菲咯啉的比色法测量组织非血红素铁含量
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Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
Please note that all translations are automatically generated.
Click here for the English version.
使用基于菲咯啉的比色法测量组织非血红素铁含量
DOI:
10.3791/63469-v
•
05:08 min
•
January 31, 2022
•
Tiago L. Duarte*
1,2
,
João V. Neves*
1,3,4
1
i3S – Instituto de Investigação e Inovação em Saúde
,
Universidade do Porto
,
2
Basic and Clinical Research on Iron Biology, IBMC – Instituto de Biologia Molecular e Celular
,
Universidade do Porto
,
3
Iron and Innate Immunity, IBMC – Instituto de Biologia Molecular e Celular
,
Universidade do Porto
,
4
ICBAS – Instituto de Ciências Biomédicas Abel Salazar
,
Universidade do Porto
Chapters
00:04
Introduction
00:49
Sample Drying
01:33
Sample Acidic Digestion
02:11
Color Development and Absorbance Reading
02:36
Results: Cuvette Versus 96-Well Microplate Comparison, Measurement of Non-Heme Iron Levels in a Mouse Model of Genetic Hemochromatosis, in Sea Bass Liver, and Whole
Drosophila melanogaster
04:41
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,提供了一种测量动物组织中非血红素铁含量的方案,使用一种简单,成熟的比色法,可以在大多数实验室中轻松实施。
Tags
Bathophenanthroline-based Colorimetric Assay
Microplate Format
Iron Overload
Iron Deficiency
Acid Extraction
Chromogen Reaction
Spectrophotometric Measurement
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