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Journal
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Biology
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蓝藻细胞外囊泡的分离和表征
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isolation and Characterization of Cyanobacterial Extracellular Vesicles
Please note that all translations are automatically generated.
Click here for the English version.
蓝藻细胞外囊泡的分离和表征
DOI:
10.3791/63481-v
•
08:44 min
•
February 03, 2022
•
Steven J. Biller*
1
,
María del Carmen Muñoz-Marín*
2
,
Steeve Lima
4,5
,
Jorge Matinha-Cardoso
4
,
Paula Tamagnini
4,6
,
Paulo Oliveira
4,6
1
Department of Biological Sciences
,
Wellesley College
,
2
Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional, Agroalimentario
,
Universidad de Córdoba
,
3
i3S – Instituto de Investigação e Inovação em Saúde
,
Universidade do Porto
,
4
IBMC – Instituto de Biologia Molecular e Celular
,
Universidade do Porto
,
5
MCbiology Doctoral Program, ICBAS – Instituto de Ciências Biomédicas Abel Salazar
,
Universidade do Porto
,
6
Departamento de Biologia, Faculdade de Ciências
,
Universidade do Porto
Chapters
00:05
Introduction
00:38
Concentrating the Vesicle Sample
02:58
Vesicle Isolation and Purification
04:47
Characterization of the Isolated Vesicles Using Transmission Electron Microscopy
05:22
Characterization of the Isolated Vesicles Using Nanoparticle Tracking Analysis
06:38
Results: An Overview of the Cyanobacterial Vesicle Isolation
07:42
Conclusion
Summary
Automatic Translation
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Automatic Translation
本方案为从蓝藻培养物中分离、浓缩和表征细胞外囊泡提供了详细的描述。还讨论了从不同尺度的培养物中纯化囊泡的方法,方法之间的权衡以及使用现场样品的注意事项。
Tags
Cyanobacteria
Extracellular Vesicles
Purification
Ultrafiltration
Tangential Flow Filtration
Concentration
Environmental Samples
Field Samples
Culture
Protocol
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